VAN ERMENGEM'S METHOD OF STAINING FLAGELLA: A MODIFICATION.

Staining bacterial flagella easily.

A wet-mount technique for staining bacterial flagella is highly successful when a stable stain and regular slides and cover slips are used. Although not producing a permanent mount, the technique is simple for routine use when the number and arrangement of flagella are critical in identifying species of motile bacteria.

Modification of the fluorescent staining method for mycobacterial cells.

The fluorescein diacetate-ethidium bromide (FDA/EB) staining method for determining the viability of mycobacterial cells reported by Kvach and Veras was modified for applying the method to Mycobacterium leprae and Mycobacterium lepraemurium. Our method clearly differentiated green-stained cells from red-orange ones with either mycobacterial species and permitted their original fluorescence for ...

Use of malachite green for staining flagella in bacteria.

Preparations are prepared, using completely clean slides and aqueous suspensions of young bacterial agar cultures. A drop of the suspension is placed on one end of the slide; the slide is then tipped, making the suspension drain down to the opposite end (Society of American Bacteriologists, Manual of Microbiological Methods, McGraw-Hill Book Co., New York, 1957). Both the mordanting and the sta...

A modification of the silver impregnation method of staining reticular fibers.

A Modification of Van Leersum's Bloodless Method for Recording Blood Pressures in Animals

It has been shown that enzymes in serum will change maltose, dextrin, and starch so that they will react as dextrose in media. These enzymes are destroyed by heating to 60 degrees C. for 15 minutes, but they are present in sera that have been refrigerated for as long as 18 months. The practice of using carbohydrate media containing unheated serum should be discouraged, and if it is used the pos...